› 3wwc

X-ray diffraction
1.49Å resolution

The complex of pOPH_S172A of pNPB

Released:
DOI: 10.2210/pdb3wwc/pdb
PDB entry 3wwc coloured by chain, front view.
PDB entry 3wwc coloured by chain, side view.
PDB entry 3wwc coloured by chain, top view.
Source organism: Pseudomonas sp. VM15C
Primary publication:
Roles of tryptophan residue and disulfide bond in the variable lid region of oxidized polyvinyl alcohol hydrolase.
Yang Y, Ko TP, Liu L, Li J, Huang CH, Chen J, Guo RT, Du G
Biochem Biophys Res Commun 452 509-14 (2014)
PMID: 25173935

Function and Biology Details

Reaction catalysed: 
Nonane-4,6-dione + H(2)O = pentan-2-one + butanoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidized polyvinyl alcohol hydrolase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 39.25 KDa
Source organism: Pseudomonas sp. VM15C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LCQ7 (Residues: 30-379; Coverage: 98%)
Gene name: pvaB
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand BUA 2 x BUA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 58.326Å b: 65.236Å c: 84.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases.
Yang et al. (2023)