› 3wxx

X-ray diffraction
2.7Å resolution

Crystal Structure of a T3SS complex from Aeromonas hydrophila

Released:
DOI: 10.2210/pdb3wxx/pdb
PDB entry 3wxx coloured by chain, front view.
PDB entry 3wxx coloured by chain, side view.
PDB entry 3wxx coloured by chain, top view.
Source organism: Aeromonas hydrophila
Primary publication:
Structure of AcrH-AopB Chaperone-Translocator Complex Reveals a Role for Membrane Hairpins in Type III Secretion System Translocon Assembly.
Nguyen VS, Jobichen C, Tan KW, Tan YW, Chan SL, Ramesh K, Yuan Y, Hong Y, Seetharaman J, Leung KY, Sivaraman J, Mok YK
Structure 23 2022-31 (2015)
PMID: 26439768

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180377 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AcrH Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 151 amino acids
Theoretical weight: 16.89 KDa
Source organism: Aeromonas hydrophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6TLM1 (Residues: 9-159; Coverage: 92%)
Gene name: acrH
Structure domains: Tetratricopeptide repeat domain
Translocator protein BipB-like C-terminal domain-containing protein Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 219 amino acids
Theoretical weight: 23.24 KDa
Source organism: Aeromonas hydrophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6TLM0 (Residues: 45-263; Coverage: 63%)
Gene name: aopB
Sequence domains: Secretion system effector C (SseC) like family

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 64 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 124.662Å b: 151.942Å c: 106.253Å
α: 90° β: 104.95° γ: 90°
R-values:
R R work R free
0.215 0.213 0.252
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The type III secretion system needle, tip, and translocon.
Dey et al. (2019)
7 more

6 mentions without citation

Molecular Targets and Strategies for Inhibition of the Bacterial Type III Secretion System (T3SS); Inhibitors Directly Binding to T3SS Components.
Hotinger et al. (2021)
5 more