› 3wyd

X-ray diffraction
1.53Å resolution

C-terminal esterase domain of LC-Est1

Released:
DOI: 10.2210/pdb3wyd/pdb
PDB entry 3wyd coloured by chain, front view.
PDB entry 3wyd coloured by chain, side view.
PDB entry 3wyd coloured by chain, top view.
Source organism: uncultured organism
Primary publication:
Structural and biochemical characterization of a metagenome-derived esterase with a long N-terminal extension.
Okano H, Hong X, Kanaya E, Angkawidjaja C, Kanaya S
Protein Sci 24 93-104 (2015)
PMID: 25348365

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.14 KDa
Source organism: uncultured organism
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0A6YVN5 (Residues: 1-208; Coverage: 100%)
Sequence domains: Prolyl oligopeptidase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 45.861Å b: 55.446Å c: 156.275Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.225
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Metagenomics: novel enzymes from non-culturable microbes.
Berini et al. (2017)
4 other articles

4 mentions without citation

Structural insight into a CE15 esterase from the marine bacterial metagenome.
De Santi et al. (2017)
3 more