› 3wzi

X-ray diffraction
2.9Å resolution

Crystal structure of AfCsx3 in complex with ssRNA

Released:
DOI: 10.2210/pdb3wzi/pdb
PDB entry 3wzi coloured by chain, front view.
PDB entry 3wzi coloured by chain, side view.
PDB entry 3wzi coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Crystal structures of CRISPR-associated Csx3 reveal a manganese-dependent deadenylation exoribonuclease.
Yan X, Guo W, Yuan YA
RNA Biol 12 749-60 (2015)
PMID: 26106927

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128021 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Uncharacterized protein AF_1864 Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.44 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28415 (Residues: 1-104; Coverage: 100%)
Gene name: AF_1864
Sequence domains: CRISPR-associated protein (Cas_csx3)
ssRNA Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 1.26 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I212121
Unit cell:
a: 71.168Å b: 80.294Å c: 181.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

RNA damage in biological conflicts and the diversity of responding RNA repair systems.
Burroughs et al. (2016)
11 other articles

5 mentions without citation

CBASS Immunity Uses CARF-Related Effectors to Sense 3'-5'- and 2'-5'-Linked Cyclic Oligonucleotide Signals and Protect Bacteria from Phage Infection.
Lowey et al. (2020)
4 more