› 3wzo

X-ray diffraction
1.5Å resolution

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution

Released:
DOI: 10.2210/pdb3wzo/pdb
Model geometry
Fit model/data
PDB entry 3wzo coloured by chain, front view.
PDB entry 3wzo coloured by chain, side view.
PDB entry 3wzo coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.
Kawato T, Mizohata E, Shimizu Y, Meshizuka T, Yamamoto T, Takasu N, Matsuoka M, Matsumura H, Kodama T, Kanai M, Doi H, Inoue T, Sugiyama A
J Biochem 157 467-75 (2015)
PMID: 25645976

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 13.23 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

4 bound ligands:
Ligand ZOE 4 x ZOE
Ligand GOL 3 x GOL
Ligand CD 9 x CD
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 55.241Å b: 85.461Å c: 86.115Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ligand-targeted theranostic nanomedicines against cancer.
Yao et al. (2016)
1 more

1 mention without citation

The intrinsically disordered SARS-CoV-2 nucleoprotein in dynamic complex with its viral partner nsp3a.
Bessa et al. (2022)