› 3x1o

X-ray diffraction
2.2Å resolution

Crystal structure of the ROQ domain of human Roquin

Released:
DOI: 10.2210/pdb3x1o/pdb
PDB entry 3x1o coloured by chain, front view.
PDB entry 3x1o coloured by chain, side view.
PDB entry 3x1o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.
Srivastava M, Duan G, Kershaw NJ, Athanasopoulos V, Yeo JH, Ose T, Hu D, Brown SH, Jergic S, Patel HR, Pratama A, Richards S, Verma A, Jones EY, Heissmeyer V, Preiss T, Dixon NE, Chong MM, Babon JJ, Vinuesa CG
Nat Commun 6 6253 (2015)
PMID: 25697406

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roquin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TC82 (Residues: 145-344; Coverage: 18%)
Gene names: KIAA2025, RC3H1, RNF198
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

1 bound ligand:
Ligand IOD 21 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 55.003Å b: 78.322Å c: 184.075Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.252
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Follicular Helper T Cells.
Vinuesa et al. (2016)
21 more

1 mention without citation

New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Zhang et al. (2015)