Function and Biology Details
Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 1
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.02 KDa
Source organism: Porcine transmissible gastroenteritis coronavirus strain Purdue
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Replicase polyprotein N-term from Coronavirus nsp1
Structure domains: Replicase polyprotein 1a
Length: 113 amino acids
Theoretical weight: 13.02 KDa
Source organism: Porcine transmissible gastroenteritis coronavirus strain Purdue
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
P0C6V2 (Residues: 1-105; Coverage: 3%)
Sequence domains: Replicase polyprotein N-term from Coronavirus nsp1
Structure domains: Replicase polyprotein 1a
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID23-2, ESRF BEAMLINE ID14-4
Spacegroup:
P1
Expression system: Escherichia coli BL21
