› 3zde

X-ray diffraction
2.45Å resolution

Potassium free structure of E. coli ExoIX

Released:
DOI: 10.2210/pdb3zde/pdb
PDB entry 3zde coloured by chain, front view.
PDB entry 3zde coloured by chain, side view.
PDB entry 3zde coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of Escherichia coli ExoIX--implications for DNA binding and catalysis in flap endonucleases.
Anstey-Gilbert CS, Hemsworth GR, Flemming CS, Hodskinson MR, Zhang J, Sedelnikova SE, Stillman TJ, Sayers JR, Artymiuk PJ
Nucleic Acids Res 41 8357-67 (2013)
PMID: 23821668

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flap endonuclease Xni Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 28.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X6R9 (Residues: 1-251; Coverage: 100%)
Gene names: ECs3658, Z4115, xni, ygdG
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 128.451Å b: 37.432Å c: 66.698Å
α: 90° β: 117.41° γ: 90°
R-values:
R R work R free
0.177 0.172 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Comprehensive classification of the PIN domain-like superfamily.
Matelska et al. (2017)
5 more