› 3zfk

X-ray diffraction
1.7Å resolution

N-terminal truncated Nuclease Domain of Colicin E7

Released:
DOI: 10.2210/pdb3zfk/pdb
PDB entry 3zfk coloured by chain, front view.
PDB entry 3zfk coloured by chain, side view.
PDB entry 3zfk coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study.
Czene A, Tóth E, Németh E, Otten H, Poulsen JC, Christensen HE, Rulíšek L, Nagata K, Larsen S, Gyurcsik B
Metallomics 6 2090-9 (2014)
PMID: 25179124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.32 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47112 (Residues: 450-573; Coverage: 22%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand SO4 9 x SO4
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P32
Unit cell:
a: 55.4Å b: 55.4Å c: 73.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Intrinsic protein disorder could be overlooked in cocrystallization conditions: An SRCD case study.
Németh et al. (2016)
1 more