› 3zgo

X-ray diffraction
1.63Å resolution

Re-refined structure of the human Sirt2 apoform

Released:
DOI: 10.2210/pdb3zgo/pdb
PDB entry 3zgo coloured by chain, front view.
PDB entry 3zgo coloured by chain, side view.
PDB entry 3zgo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure analysis of human Sirt2 and its ADP-ribose complex.
Moniot S, Schutkowski M, Steegborn C
J Struct Biol 182 136-43 (2013)
PMID: 23454361

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase sirtuin-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 325 amino acids
Theoretical weight: 36.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IXJ6 (Residues: 34-356; Coverage: 83%)
Gene names: SIR2L, SIR2L2, SIRT2
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 3 x ZN
Ligand P6G 1 x P6G
Ligand PGE 7 x PGE
Ligand EDO 1 x EDO
Ligand EOH 1 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: null, CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 78.94Å b: 119.07Å c: 218.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Using mitochondrial sirtuins as drug targets: disease implications and available compounds.
Gertz et al. (2016)
11 more

19 mentions without citation

Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress.
Hentchel et al. (2015)
18 more