› 3zgv

X-ray diffraction
2.27Å resolution

Structure of human SIRT2 in complex with ADP-ribose

Released:
DOI: 10.2210/pdb3zgv/pdb
PDB entry 3zgv coloured by chain, front view.
PDB entry 3zgv coloured by chain, side view.
PDB entry 3zgv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure analysis of human Sirt2 and its ADP-ribose complex.
Moniot S, Schutkowski M, Steegborn C
J Struct Biol 182 136-43 (2013)
PMID: 23454361

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase sirtuin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 36.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IXJ6 (Residues: 34-356; Coverage: 83%)
Gene names: SIR2L, SIR2L2, SIRT2
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand ACT 6 x ACT
Ligand AR6 2 x AR6
Ligand GOL 1 x GOL
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 77.86Å b: 77.98Å c: 114.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.15 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Sirtuin activators and inhibitors: Promises, achievements, and challenges.
Dai et al. (2018)
11 more

18 mentions without citation

Recent Developments in New Therapeutic Agents against Alzheimer and Parkinson Diseases: In-Silico Approaches.
Cruz-Vicente et al. (2021)
17 more