› 3zie

X-ray diffraction
2Å resolution

SepF-like protein from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb3zie/pdb
PDB entry 3zie coloured by chain, front view.
PDB entry 3zie coloured by chain, side view.
PDB entry 3zie coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring.
Duman R, Ishikawa S, Celik I, Strahl H, Ogasawara N, Troc P, Löwe J, Hamoen LW
Proc Natl Acad Sci U S A 110 E4601-10 (2013)
PMID: 24218584

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF552 domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 86 amino acids
Theoretical weight: 9.76 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29476 (Residues: 37-122; Coverage: 71%)
Gene name: AF_0782
Sequence domains: Cell division protein SepF
Structure domains: SepF-like protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 107.02Å b: 64.09Å c: 82.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Splitsville: structural and functional insights into the dynamic bacterial Z ring.
Haeusser et al. (2016)
20 more

4 mentions without citation

Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring.
Duman et al. (2013)
3 more