› 3zih

X-ray diffraction
2Å resolution

Bacillus subtilis SepF, C-terminal domain

Released:
DOI: 10.2210/pdb3zih/pdb
PDB entry 3zih coloured by chain, front view.
PDB entry 3zih coloured by chain, side view.
PDB entry 3zih coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring.
Duman R, Ishikawa S, Celik I, Strahl H, Ogasawara N, Troc P, Löwe J, Hamoen LW
Proc Natl Acad Sci U S A 110 E4601-10 (2013)
PMID: 24218584

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein SepF Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.67 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31728 (Residues: 57-151; Coverage: 63%)
Gene names: BSU15390, sepF, ylmF
Sequence domains: Cell division protein SepF
Structure domains: SepF-like protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 40.456Å b: 40.456Å c: 170.331Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.184 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Splitsville: structural and functional insights into the dynamic bacterial Z ring.
Haeusser et al. (2016)
19 more

3 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
2 more