› 3zii

X-ray diffraction
2.2Å resolution

Bacillus subtilis SepF G109K, C-terminal domain

Released:
DOI: 10.2210/pdb3zii/pdb
PDB entry 3zii coloured by chain, front view.
PDB entry 3zii coloured by chain, side view.
PDB entry 3zii coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring.
Duman R, Ishikawa S, Celik I, Strahl H, Ogasawara N, Troc P, Löwe J, Hamoen LW
Proc Natl Acad Sci U S A 110 E4601-10 (2013)
PMID: 24218584

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein SepF Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.75 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31728 (Residues: 57-151; Coverage: 63%)
Gene names: BSU15390, sepF, ylmF
Sequence domains: Cell division protein SepF
Structure domains: SepF-like protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 42.01Å b: 80.14Å c: 51.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Splitsville: structural and functional insights into the dynamic bacterial Z ring.
Haeusser et al. (2016)
20 more