› 3zlj

X-ray diffraction
3.1Å resolution

CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA

Released:
DOI: 10.2210/pdb3zlj/pdb
PDB entry 3zlj coloured by chain, front view.
PDB entry 3zlj coloured by chain, side view.
PDB entry 3zlj coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation.
Groothuizen FS, Fish A, Petoukhov MV, Reumer A, Manelyte L, Winterwerp HH, Marinus MG, Lebbink JH, Svergun DI, Friedhoff P, Sixma TK
Nucleic Acids Res 41 8166-81 (2013)
PMID: 23821665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-218955 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 89.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
DNA mismatch repair protein MutS Chains: C, D
Molecule details ›
Chains: C, D
Length: 53 amino acids
Theoretical weight: 5.81 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23909 (Residues: 801-853; Coverage: 6%)
Gene names: JW2703, b2733, fdv, mutS
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP*CP*AP*CP*CP *AP*GP*TP*GP*TP*CP*AP)-3' Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.43 KDa
5'-D(*TP*GP*AP*CP*AP*CP*TP*GP*GP*TP*GP*CP*TP*TP *GP*GP*CP*AP*GP*CP*TP)-3' Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.47 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 110.288Å b: 91.152Å c: 112.855Å
α: 90° β: 101.79° γ: 90°
R-values:
R R work R free
0.229 0.227 0.263
Expression system: Escherichia coli B

Quick links

Citations

2 review citations

Eukaryotic Mismatch Repair in Relation to DNA Replication.
Kunkel et al. (2015)
1 more

2 mentions without citation

Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation.
Groothuizen et al. (2013)
1 more