› 3zn8

Electron Microscopy
12Å resolution

Structural Basis of Signal Sequence Surveillance and Selection by the SRP-SR Complex

Released:
DOI: 10.2210/pdb3zn8/pdb
PDB entry 3zn8 coloured by chain, front view.
PDB entry 3zn8 coloured by chain, side view.
PDB entry 3zn8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex.
von Loeffelholz O, Knoops K, Ariosa A, Zhang X, Karuppasamy M, Huard K, Schoehn G, Berger I, Shan SO, Schaffitzel C
Nat Struct Mol Biol 20 604-10 (2013)
PMID: 23563142
Related structures: EMD-2316

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-301040 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 32.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O07347 (Residues: 2-295; Coverage: 68%)
Gene name: ffh
Sequence domains:
Signal recognition particle receptor FtsY Chain: D
Molecule details ›
Chain: D
Length: 295 amino acids
Theoretical weight: 32.18 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10121 (Residues: 201-495; Coverage: 59%)
Gene names: JW3429, b3464, ftsY
Sequence domains:
Signal recognition particle 54 kDa protein Chain: M
Dipeptidyl aminopeptidase B Chain: S
4.5 S RNA Chain: G

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 12Å
Relevant EMDB volumes: EMD-2316
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Fidelity of cotranslational protein targeting by the signal recognition particle.
Zhang et al. (2014)
3 more

1 mention without citation

Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex.
von Loeffelholz et al. (2013)