3znj

X-ray diffraction
2.1Å resolution

Crystal structure of unliganded ClcF from R.opacus 1CP in crystal form 1.

Released:

Function and Biology Details

Reaction catalysed:
(+)-muconolactone = 5-oxo-4,5-dihydrofuran-2-acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo decamer (preferred)
PDBe Complex ID:
PDB-CPX-184469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Muconolactone Delta-isomerase Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f
Length: 94 amino acids
Theoretical weight: 11.21 KDa
Source organism: Rhodococcus opacus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G9L0 (Residues: 1-94; Coverage: 100%)
Gene names: R1CP_39625, clcF
Sequence domains: Muconolactone delta-isomerase
Structure domains: Dimeric alpha+beta barrel

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 151.14Å b: 79.43Å c: 198.97Å
α: 90° β: 93.41° γ: 90°
R-values:
R R work R free
0.164 0.164 0.224
Expression system: Escherichia coli