› 3zo0

X-ray diffraction
1.99Å resolution

Mouse IgG2a in complex with mouse TRIM21 PRYSPRY

Released:
DOI: 10.2210/pdb3zo0/pdb
PDB entry 3zo0 coloured by chain, front view.
PDB entry 3zo0 coloured by chain, side view.
PDB entry 3zo0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
TRIM21 is an IgG receptor that is structurally, thermodynamically, and kinetically conserved.
Keeble AH, Khan Z, Forster A, James LC
Proc Natl Acad Sci U S A 105 6045-50 (2008)
PMID: 18420815

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134902 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig gamma-2A chain C region, A allele Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.85 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01863 (Residues: 120-327; Coverage: 63%)
Gene name: Ighg
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
E3 ubiquitin-protein ligase TRIM21 Chain: B
Molecule details ›
Chain: B
Length: 182 amino acids
Theoretical weight: 20.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62191 (Residues: 291-470; Coverage: 38%)
Gene names: Ro52, Ssa1, Trim21
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA, GAL
Carbohydrate polymer
2 bound ligands:
Ligand MAN 1 x MAN
Ligand FUC 1 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 45.458Å b: 186.185Å c: 124.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.181 0.253
Expression system: Escherichia coli

Quick links

Citations

45 review citations

Horror autoinflammaticus: the molecular pathophysiology of autoinflammatory disease (*).
Masters et al. (2009)
44 more

3 mentions without citation

Efficient Generation of Bispecific Murine Antibodies for Pre-Clinical Investigations in Syngeneic Rodent Models.
Labrijn et al. (2017)
2 more