› 3zpv

X-ray diffraction
2.68Å resolution

Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain

Released:
DOI: 10.2210/pdb3zpv/pdb
PDB entry 3zpv coloured by chain, front view.
PDB entry 3zpv coloured by chain, side view.
PDB entry 3zpv coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Evolutionary adaptation of the fly Pygo PHD finger toward recognizing histone H3 tail methylated at arginine 2.
Miller TC, Mieszczanek J, Sánchez-Barrena MJ, Rutherford TJ, Fiedler M, Bienz M
Structure 21 2208-20 (2013)
PMID: 24183574

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188293 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein BCL9 homolog Chains: 0, 2, 4, 6, 8, B, D, F, H, J, L, N, P, R, T, V, X, Z
Molecule details ›
Chains: 0, 2, 4, 6, 8, B, D, F, H, J, L, N, P, R, T, V, X, Z
Length: 37 amino acids
Theoretical weight: 3.99 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q961D9 (Residues: 321-353; Coverage: 2%)
Gene names: BCL9, CG2041, lgs
Protein pygopus Chains: 1, 3, 5, 7, 9, A, C, G, I, K, M, Q, S, U, W
Molecule details ›
Chains: 1, 3, 5, 7, 9, A, C, G, I, K, M, Q, S, U, W
Length: 62 amino acids
Theoretical weight: 6.96 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V9W8 (Residues: 747-804; Coverage: 7%)
Gene names: CG11518, gam, pygo
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Protein pygopus Chains: E, O, Y
Molecule details ›
Chains: E, O, Y
Length: 62 amino acids
Theoretical weight: 6.97 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V9W8 (Residues: 747-804; Coverage: 7%)
Gene names: CG11518, gam, pygo
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 36 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 105.21Å b: 111.96Å c: 190.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Fuzzy Drug Targets: Disordered Proteins in the Drug-Discovery Realm.
Saurabh et al. (2023)
7 other articles

1 mention without citation

The structural biology of canonical Wnt signalling.
Agostino et al. (2020)