› 3zqi

X-ray diffraction
1.5Å resolution

Structure of Tetracycline repressor in complex with inducer peptide- TIP2

Released:
DOI: 10.2210/pdb3zqi/pdb
PDB entry 3zqi coloured by chain, front view.
PDB entry 3zqi coloured by chain, side view.
PDB entry 3zqi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
An exclusive α/β code directs allostery in TetR-peptide complexes.
Sevvana M, Goetz C, Goeke D, Wimmer C, Berens C, Hillen W, Muller YA
J Mol Biol 416 46-56 (2012)
PMID: 22178479

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137869 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tetracycline repressor protein class B from transposon Tn10; Tetracycline repressor protein class D Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACT4 (Residues: 1-1, 121-121, 188-208; Coverage: 11%)
  • Canonical: P04483 (Residues: 2-120, 122-187; Coverage: 89%)
Gene name: tetR
Sequence domains:
Structure domains:
INDUCER PEPTIDE TIP2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 2.03 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand EDO 5 x EDO
Ligand MG 1 x MG
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 56.7Å b: 78.56Å c: 103.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.195
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

The TetR family of regulators.
Cuthbertson et al. (2013)
8 other articles

2 mentions without citation

Engineering DNA recognition and allosteric response properties of TetR family proteins by using a module-swapping strategy.
Dimas et al. (2019)
1 more