› 3zrr

X-ray diffraction
1.99Å resolution

Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb3zrr/pdb
PDB entry 3zrr coloured by chain, front view.
PDB entry 3zrr coloured by chain, side view.
PDB entry 3zrr coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus.
Sayer C, Bommer M, Isupov M, Ward J, Littlechild J
Acta Crystallogr D Biol Crystallogr 68 763-72 (2012)
PMID: 22751661

Function and Biology Details

Reaction catalysed: 
L-serine + pyruvate = 3-hydroxypyruvate + L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class V domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 384 amino acids
Theoretical weight: 42.03 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97VM5 (Residues: 1-384; Coverage: 100%)
Gene names: SSO2597, agxT
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PXG 2 x PXG
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS
Spacegroup: C2
Unit cell:
a: 160.835Å b: 55.205Å c: 102.32Å
α: 90° β: 111.95° γ: 90°
R-values:
R R work R free
0.189 0.186 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Enzymes from Extreme Environments and Their Industrial Applications.
Littlechild JA. (2015)
3 more