› 3zt9

X-ray diffraction
1.75Å resolution

The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling

Released:
DOI: 10.2210/pdb3zt9/pdb
PDB entry 3zt9 coloured by chain, front view.
PDB entry 3zt9 coloured by chain, side view.
PDB entry 3zt9 coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling.
Quin MB, Berrisford JM, Newman JA, Baslé A, Lewis RJ, Marles-Wright J
Structure 20 350-63 (2012)
PMID: 22325782

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PPM-type phosphatase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 20.8 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q2RIF7 (Residues: 1-193; Coverage: 100%)
Gene name: Moth_1472
Sequence domains: Stage II sporulation protein E (SpoIIE)
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
Ligand MN 3 x MN
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 42Å b: 46.92Å c: 87.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.189
Expression system: Escherichia coli B

Quick links

Citations

1 review citation

Activation of the General Stress Response of Bacillus subtilis by Visible Light.
van der Steen et al. (2015)
17 other articles

3 mentions without citation

"PP2C7s", Genes Most Highly Elaborated in Photosynthetic Organisms, Reveal the Bacterial Origin and Stepwise Evolution of PPM/PP2C Protein Phosphatases.
Kerk et al. (2015)
2 more