› 3ztn

X-ray diffraction
3Å resolution

STRUCTURE OF INFLUENZA A NEUTRALIZING ANTIBODY SELECTED FROM CULTURES OF SINGLE HUMAN PLASMA CELLS IN COMPLEX WITH HUMAN H1 INFLUENZA HAEMAGGLUTININ.

Released:
DOI: 10.2210/pdb3ztn/pdb
PDB entry 3ztn coloured by chain, front view.
PDB entry 3ztn coloured by chain, side view.
PDB entry 3ztn coloured by chain, top view.
Source organisms:
Primary publication:
A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins.
Corti D, Voss J, Gamblin SJ, Codoni G, Macagno A, Jarrossay D, Vachieri SG, Pinna D, Minola A, Vanzetta F, Silacci C, Fernandez-Rodriguez BM, Agatic G, Bianchi S, Giacchetto-Sasselli I, Calder L, Sallusto F, Collins P, Haire LF, Temperton N, Langedijk JP, Skehel JJ, Lanzavecchia A
Science 333 850-6 (2011)
PMID: 21798894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207713 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.39 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
UniProt:
  • Canonical: C3W5S1 (Residues: 18-344; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.13 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
UniProt:
  • Canonical: C3W5S1 (Residues: 345-520; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
FI6V3 ANTIBODY LIGHT CHAIN Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
FI6V3 ANTIBODY LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 24.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 5 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4132
Unit cell:
a: 206.1Å b: 206.1Å c: 206.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.214 0.289
Expression system: Spodoptera frugiperda

Quick links

Citations

222 review citations

The promise and challenge of high-throughput sequencing of the antibody repertoire.
Georgiou et al. (2014)
221 more

32 mentions without citation

Structural insights into key sites of vulnerability on HIV-1 Env and influenza HA.
Julien et al. (2012)
31 more