› 3zwe

X-ray diffraction
1.75Å resolution

Structure of BambL, a lectin from Burkholderia ambifaria, complexed with blood group B epitope

Released:
DOI: 10.2210/pdb3zwe/pdb
PDB entry 3zwe coloured by chain, front view.
PDB entry 3zwe coloured by chain, side view.
PDB entry 3zwe coloured by chain, top view.
Source organism: Burkholderia ambifaria AMMD
Primary publication:
Fucose-binding lectin from opportunistic pathogen Burkholderia ambifaria binds to both plant and human oligosaccharidic epitopes.
Audfray A, Claudinon J, Abounit S, Ruvoën-Clouet N, Larson G, Smith DF, Wimmerová M, Le Pendu J, Römer W, Varrot A, Imberty A
J Biol Chem 287 4335-47 (2012)
PMID: 22170069

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Fucose-binding lectin protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 87 amino acids
Theoretical weight: 9.39 KDa
Source organism: Burkholderia ambifaria AMMD
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0B4G1 (Residues: 1-87; Coverage: 100%)
Gene name: Bamb_5415
Sequence domains: Fungal fucose-specific lectin
Structure domains: Lipocalin

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GAL, BGC, GLA
Carbohydrate polymer
1 bound ligand:
Ligand FUC 3 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 47.98Å b: 49.14Å c: 102.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Methods for 2-Deoxyglycoside Synthesis.
Bennett et al. (2018)
7 more

5 mentions without citation

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
4 more