› 3zwj

X-ray diffraction
2.37Å resolution

CRYSTAL STRUCTURE OF THE PORE-FORMING TOXIN FRAC FROM ACTINIA FRAGACEA (Form 3)

Released:
DOI: 10.2210/pdb3zwj/pdb
PDB entry 3zwj coloured by chain, front view.
PDB entry 3zwj coloured by chain, side view.
PDB entry 3zwj coloured by chain, top view.
Source organism: Actinia fragacea
Primary publication:
Pores of the toxin FraC assemble into 2D hexagonal clusters in both crystal structures and model membranes.
Mechaly AE, Bellomio A, Morante K, Agirre J, Gil-Cartón D, Valle M, González-Mañas JM, Guérin DM
J Struct Biol 180 312-7 (2012)
PMID: 22728830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DELTA-actitoxin-Afr1a Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 179 amino acids
Theoretical weight: 19.75 KDa
Source organism: Actinia fragacea
UniProt:
  • Canonical: B9W5G6 (Residues: 1-179; Coverage: 100%)
Sequence domains: Sea anemone cytotoxic protein
Structure domains: Cytolysin/lectin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P321
Unit cell:
a: 117.969Å b: 117.969Å c: 256.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.192 0.215

Quick links

Citations

3 review citations

Functional and Structural Variation among Sticholysins, Pore-Forming Proteins from the Sea Anemone Stichodactyla helianthus.
Rivera-de-Torre et al. (2020)
2 more

1 mention without citation

Pore-forming moss protein bryoporin is structurally and mechanistically related to actinoporins from evolutionarily distant cnidarians.
Šolinc et al. (2022)