› 3zx5

X-ray diffraction
1.81Å resolution

The 3-dimensional structure of MpgP from Thermus thermophilus HB27, covalently bound to vanadate and in complex with alpha- mannosylglycerate and magnesium

Released:
DOI: 10.2210/pdb3zx5/pdb
PDB entry 3zx5 coloured by chain, front view.
PDB entry 3zx5 coloured by chain, side view.
PDB entry 3zx5 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Three-dimensional structure of mannosyl-3-phosphoglycerate phosphatase from Thermus thermophilus HB27: a new member of the haloalcanoic acid dehalogenase superfamily.
Gonçalves S, Esteves AM, Santos H, Borges N, Matias PM
Biochemistry 50 9551-67 (2011)
PMID: 21961705

Function and Biology Details

Reaction catalysed: 
2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H(2)O = 2-O-(alpha-D-mannosyl)-D-glycerate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosyl-3-phosphoglycerate phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 28.22 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72K29 (Residues: 1-259; Coverage: 100%)
Gene name: TT_C0589
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand VN4 2 x VN4
Ligand 2M8 2 x 2M8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 39.948Å b: 71.263Å c: 91.217Å
α: 90° β: 95.52° γ: 90°
R-values:
R R work R free
0.17 0.168 0.213
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Mannosylglycerate: structural analysis of biosynthesis and evolutionary history.
Borges et al. (2014)
1 more