› 3zzm

X-ray diffraction
2.2Å resolution

Crystal structure of Mycobacterium tuberculosis PurH with a novel bound nucleotide CFAIR, at 2.2 A resolution.

Released:
DOI: 10.2210/pdb3zzm/pdb
PDB entry 3zzm coloured by chain, front view.
PDB entry 3zzm coloured by chain, side view.
PDB entry 3zzm coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural analyses of a purine biosynthetic enzyme from Mycobacterium tuberculosis reveal a novel bound nucleotide.
Le Nours J, Bulloch EM, Zhang Z, Greenwood DR, Middleditch MJ, Dickson JM, Baker EN
J Biol Chem 286 40706-16 (2011)
PMID: 21956117

Function and Biology Details

Reactions catalysed: 
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional purine biosynthesis protein PurH Chains: A, B
Molecule details ›
Chains: A, B
Length: 523 amino acids
Theoretical weight: 55.08 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHM7 (Residues: 1-523; Coverage: 100%)
Gene names: MTCY10D7.17c, Rv0957, purH
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand K 2 x K
Ligand JLN 1 x JLN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 76.594Å b: 108.22Å c: 130.634Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Nucleoside analogs and tuberculosis: new weapons against an old enemy.
Ferrari et al. (2015)
3 other articles

4 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
3 more