› 3zzq

X-ray diffraction
1.75Å resolution

Engineered 12-subunit Bacillus subtilis trp RNA-binding attenuation protein (TRAP)

Released:
DOI: 10.2210/pdb3zzq/pdb
PDB entry 3zzq coloured by chain, front view.
PDB entry 3zzq coloured by chain, side view.
PDB entry 3zzq coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism.
Chen CS, Smits C, Dodson GG, Shevtsov MB, Merlino N, Gollnick P, Antson AA
PLoS One 6 e25296 (2011)
PMID: 21984911

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription attenuation protein MtrB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 65 amino acids
Theoretical weight: 7.14 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19466 (Residues: 7-71; Coverage: 87%)
Gene names: BSU22770, mtrB
Sequence domains: Tryptophan RNA-binding attenuator protein
Structure domains: TRAP-like

Ligands and Environments

1 bound ligand:
Ligand TRP 12 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I23
Unit cell:
a: 146.348Å b: 146.348Å c: 146.348Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.19
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Simple mechanisms for the evolution of protein complexity.
Pillai et al. (2022)
6 other articles

1 mention without citation

How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism.
Chen et al. (2011)