› 4al9

X-ray diffraction
1.75Å resolution

Crystal structure of the lectin PA-IL from Pseudomonas aeruginoas in complex with melibiose

Released:
DOI: 10.2210/pdb4al9/pdb
PDB entry 4al9 coloured by chain, front view.
PDB entry 4al9 coloured by chain, side view.
PDB entry 4al9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Secondary sugar binding site identified for LecA lectin from Pseudomonas aeruginosa.
Blanchard B, Imberty A, Varrot A
Proteins 82 1060-5 (2014)
PMID: 24123124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PA-I galactophilic lectin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 121 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05097 (Residues: 2-122; Coverage: 99%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: GLA, GLC
Carbohydrate polymer
3 bound ligands:
Ligand CA 8 x CA
Ligand GLA 6 x GLA
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 50.07Å b: 58.13Å c: 75.94Å
α: 101.13° β: 92.89° γ: 100.98°
R-values:
R R work R free
0.169 0.167 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Biofilm Matrix Proteins.
Fong et al. (2015)
2 more

4 mentions without citation

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers.
de Boer et al. (2019)
3 more