› 4b4q

X-ray diffraction
2Å resolution

Crystal Structure of the lectin domain of F18 fimbrial adhesin FedF in complex with blood group A type 1 hexasaccharide

Released:
DOI: 10.2210/pdb4b4q/pdb
PDB entry 4b4q coloured by chain, front view.
PDB entry 4b4q coloured by chain, side view.
PDB entry 4b4q coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF.
Moonens K, Bouckaert J, Coddens A, Tran T, Panjikar S, De Kerpel M, Cox E, Remaut H, De Greve H
Mol Microbiol 86 82-95 (2012)
PMID: 22812428

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
F18 fimbrial adhesin AC Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 16.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47212 (Residues: 35-185; Coverage: 54%)
Gene name: fedF
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL, NAG, FUC, A2G
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 35.16Å b: 54.55Å c: 145.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Animal Enterotoxigenic Escherichia coli.
Dubreuil et al. (2016)
5 more

2 mentions without citation

Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis.
Fang et al. (2021)
1 more