› 4a25

X-ray diffraction
2Å resolution

X-ray structure Dps from Kineococcus radiotolerans in complex with Mn (II) ions.

Released:
DOI: 10.2210/pdb4a25/pdb
PDB entry 4a25 coloured by chain, front view.
PDB entry 4a25 coloured by chain, side view.
PDB entry 4a25 coloured by chain, top view.
Source organism: Kineococcus radiotolerans
Primary publication:
Kineococcus radiotolerans Dps forms a heteronuclear Mn-Fe ferroxidase center that may explain the Mn-dependent protection against oxidative stress.
Ardini M, Fiorillo A, Fittipaldi M, Stefanini S, Gatteschi D, Ilari A, Chiancone E
Biochim Biophys Acta 1830 3745-55 (2013)
PMID: 23396000

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin/DPS protein domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 169 amino acids
Theoretical weight: 18.52 KDa
Source organism: Kineococcus radiotolerans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6WG04 (Residues: 2-170; Coverage: 99%)
Gene name: Krad_4280
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand MN 10 x MN
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: R3
Unit cell:
a: 150.474Å b: 150.474Å c: 86.618Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Manganese acquisition and homeostasis at the host-pathogen interface.
Lisher et al. (2013)
4 more