› 4a35

X-ray diffraction
1.74Å resolution

Crystal structure of human Mitochondrial enolase superfamily member 1 (ENOSF1)

Released:
DOI: 10.2210/pdb4a35/pdb
PDB entry 4a35 coloured by chain, front view.
PDB entry 4a35 coloured by chain, side view.
PDB entry 4a35 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Enzymatic and structural characterization of rTSγ provides insights into the function of rTSβ.
Wichelecki DJ, Froese DS, Kopec J, Muniz JR, Yue WW, Gerlt JA
Biochemistry 53 2732-8 (2014)
PMID: 24697329

Function and Biology Details

Reaction catalysed: 
L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial enolase superfamily member 1 Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 49.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7L5Y1 (Residues: 1-440; Coverage: 99%)
Gene names: ENOSF1, RTS, TYMSAS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6522
Unit cell:
a: 84.77Å b: 84.77Å c: 316.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
1 more

7 mentions without citation

UniProt: a hub for protein information.
UniProt Consortium. (2015)
6 more