› 4a37

X-ray diffraction
1.6Å resolution

Metallo-carboxypeptidase from Pseudomonas Aeruginosa

Released:
DOI: 10.2210/pdb4a37/pdb
PDB entry 4a37 coloured by chain, front view.
PDB entry 4a37 coloured by chain, side view.
PDB entry 4a37 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The novel structure of a cytosolic M14 metallocarboxypeptidase (CCP) from Pseudomonas aeruginosa: a model for mammalian CCPs.
Otero A, Rodríguez de la Vega M, Tanco S, Lorenzo J, Avilés FX, Reverter D
FASEB J 26 3754-64 (2012)
PMID: 22645247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M14 carboxypeptidase A domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 44.45 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I012 (Residues: 1-375; Coverage: 100%)
Gene name: PA2831
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 41.89Å b: 83.84Å c: 107.45Å
α: 90° β: 96.37° γ: 90°
R-values:
R R work R free
0.198 0.198 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Insights of the tubulin code in gametes and embryos: from basic research to potential clinical applications in humans†.
Amargant et al. (2019)
1 more

2 mentions without citation

The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational removal of acidic amino acids.
Tort et al. (2014)
1 more