› 4a3s

X-ray diffraction
2.3Å resolution

Crystal structure of PFK from Bacillus subtilis

Released:
DOI: 10.2210/pdb4a3s/pdb
PDB entry 4a3s coloured by chain, front view.
PDB entry 4a3s coloured by chain, side view.
PDB entry 4a3s coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome.
Newman JA, Hewitt L, Rodrigues C, Solovyova AS, Harwood CR, Lewis RJ
J Mol Biol 416 121-36 (2012)
PMID: 22198292

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 34.3 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O34529 (Residues: 1-319; Coverage: 100%)
Gene names: BSU29190, pfk, pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 102.429Å b: 77.007Å c: 101.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.276
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Lehnik-Habrink et al. (2012)
11 more

1 mention without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)