› 4a3y

X-ray diffraction
2.15Å resolution

Crystal structure of Raucaffricine glucosidase from ajmaline biosynthesis pathway

Released:
DOI: 10.2210/pdb4a3y/pdb
PDB entry 4a3y coloured by chain, front view.
PDB entry 4a3y coloured by chain, side view.
PDB entry 4a3y coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
Structures of alkaloid biosynthetic glucosidases decode substrate specificity.
Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stöckigt J
ACS Chem Biol 7 226-34 (2012)
PMID: 22004291

Function and Biology Details

Reactions catalysed: 
UDP-glucose + vomilenine = UDP + raucaffricine
Raucaffricine + H(2)O = D-glucose + vomilenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Raucaffricine-O-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 540 amino acids
Theoretical weight: 61 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SPP9 (Residues: 1-540; Coverage: 100%)
Gene names: RG, VGT
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 102.774Å b: 127.329Å c: 215.837Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.204
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Gates of enzymes.
Gora et al. (2013)
2 more

2 mentions without citation

Structural determinants allowing transferase activity in SENSITIVE TO FREEZING 2, classified as a family I glycosyl hydrolase.
Roston et al. (2014)
1 more