› 4a48

X-ray diffraction
1.4Å resolution

Crosstalk between Cu(I) and Zn(II) homeostasis

Released:
DOI: 10.2210/pdb4a48/pdb
PDB entry 4a48 coloured by chain, front view.
PDB entry 4a48 coloured by chain, side view.
PDB entry 4a48 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Investigating the role of zinc and copper binding motifs of trafficking sites in the cyanobacterium Synechocystis PCC 6803.
Badarau A, Baslé A, Firbank SJ, Dennison C
Biochemistry 52 6816-23 (2013)
PMID: 24050657

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Cu(+)(Side 1) = ADP + phosphate + Cu(+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable copper-transporting ATPase PacS Chains: A, B
Molecule details ›
Chains: A, B
Length: 69 amino acids
Theoretical weight: 7.4 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73241 (Residues: 2-70; Coverage: 9%)
Gene names: pacS, sll1920
Sequence domains: Heavy-metal-associated domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 24.66Å b: 52.6Å c: 39.92Å
α: 90° β: 98.13° γ: 90°
R-values:
R R work R free
0.143 0.14 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Crystal Structure of the Human Copper Chaperone ATOX1 Bound to Zinc Ion.
Mangini et al. (2022)
1 more

6 mentions without citation

A less-biased analysis of metalloproteins reveals novel zinc coordination geometries.
Yao et al. (2015)
5 more