› 4a4k

X-ray diffraction
3.25Å resolution

Crystal structure of the S. cerevisiae Ski2 insertion domain

Released:
DOI: 10.2210/pdb4a4k/pdb
PDB entry 4a4k coloured by chain, front view.
PDB entry 4a4k coloured by chain, side view.
PDB entry 4a4k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome.
Halbach F, Rode M, Conti E
RNA 18 124-34 (2012)
PMID: 22114319

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral helicase SKI2 Chains: A, C, E, G, I
Molecule details ›
Chains: A, C, E, G, I
Length: 256 amino acids
Theoretical weight: 29.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35207 (Residues: 835-1085; Coverage: 20%)
Gene names: L8084.17, SKI2, YLR398C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 230.463Å b: 123.139Å c: 153.201Å
α: 90° β: 131.05° γ: 90°
R-values:
R R work R free
0.237 0.236 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

The regulation and functions of the nuclear RNA exosome complex.
Kilchert et al. (2016)
16 more

3 mentions without citation

Ski2-like RNA helicase structures: common themes and complex assemblies.
Johnson et al. (2013)
2 more