› 4a4z

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE S. CEREVISIAE DEXH HELICASE SKI2 BOUND TO AMPPNP

Released:
DOI: 10.2210/pdb4a4z/pdb
PDB entry 4a4z coloured by chain, front view.
PDB entry 4a4z coloured by chain, side view.
PDB entry 4a4z coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome.
Halbach F, Rode M, Conti E
RNA 18 124-34 (2012)
PMID: 22114319

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral helicase SKI2 Chain: A
Molecule details ›
Chain: A
Length: 997 amino acids
Theoretical weight: 113.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35207 (Residues: 296-1287; Coverage: 77%)
Gene names: L8084.17, SKI2, YLR398C
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 82.794Å b: 118.551Å c: 129.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.275
Expression system: Trichoplusia ni

Quick links

Citations

16 review citations

RNA helicase proteins as chaperones and remodelers.
Jarmoskaite et al. (2014)
15 more

5 mentions without citation

Targeting RNA for processing or destruction by the eukaryotic RNA exosome and its cofactors.
Zinder et al. (2017)
4 more