Function and Biology Details
Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Methyltransferase/Protease/Ubiquitinyl hydrolase (preferred)
PDBe Complex ID:
PDB-CPX-145286 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methyltransferase/Protease/Ubiquitinyl hydrolase
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 17.78 KDa
Source organism: Turnip yellow mosaic virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains: Cathepsin B; Chain A
Length: 160 amino acids
Theoretical weight: 17.78 KDa
Source organism: Turnip yellow mosaic virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P10358 (Residues: 728-879; Coverage: 8%)
Structure domains: Cathepsin B; Chain A
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SOLEIL BEAMLINE PROXIMA 1
Spacegroup:
P3121
Expression system: Escherichia coli BL21(DE3)
