› 4a5y

X-ray diffraction
2.45Å resolution

Intermediate state of human kinesin Eg5 in complex with Ispinesib

Released:
DOI: 10.2210/pdb4a5y/pdb
PDB entry 4a5y coloured by chain, front view.
PDB entry 4a5y coloured by chain, side view.
PDB entry 4a5y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
"Snapshots" of ispinesib-induced conformational changes in the mitotic kinesin Eg5.
Kaan HY, Major J, Tkocz K, Kozielski F, Rosenfeld SS
J Biol Chem 288 18588-98 (2013)
PMID: 23658017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF11 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 368 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52732 (Residues: 1-368; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand ADP 3 x ADP
Ligand CL 2 x CL
Ligand G7X 3 x G7X
Ligand MG 1 x MG
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 85.63Å b: 91.93Å c: 163.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Kinesin, 30 years later: Recent insights from structural studies.
Wang et al. (2015)
15 other articles

2 mentions without citation

Why Some Targets Benefit from beyond Rule of Five Drugs.
Egbert et al. (2019)
1 more