› 4a61

X-ray diffraction
2Å resolution

ParM from plasmid R1 in complex with AMPPNP

Released:
DOI: 10.2210/pdb4a61/pdb
PDB entry 4a61 coloured by chain, front view.
PDB entry 4a61 coloured by chain, side view.
PDB entry 4a61 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.
Gayathri P, Fujii T, Møller-Jensen J, van den Ent F, Namba K, Löwe J
Science 338 1334-7 (2012)
PMID: 23112295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmid segregation protein ParM Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11904 (Residues: 2-320; Coverage: 100%)
Gene names: parM, stbA
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P4322
Unit cell:
a: 63.541Å b: 63.541Å c: 164.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

20 review citations

Subcellular Organization: A Critical Feature of Bacterial Cell Replication.
Surovtsev et al. (2018)
19 more

4 mentions without citation

Bacterial actin MreB forms antiparallel double filaments.
van den Ent et al. (2014)
3 more