› 4a6j

Electron Microscopy
7.2Å resolution

Structural model of ParM filament based on CryoEM map

Released:
DOI: 10.2210/pdb4a6j/pdb
PDB entry 4a6j coloured by chain, front view.
PDB entry 4a6j coloured by chain, side view.
PDB entry 4a6j coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.
Gayathri P, Fujii T, Møller-Jensen J, van den Ent F, Namba K, Löwe J
Science 338 1334-7 (2012)
PMID: 23112295
Related structures: EMD-1980

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmid segregation protein ParM Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 320 amino acids
Theoretical weight: 35.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11904 (Residues: 1-320; Coverage: 100%)
Gene names: parM, stbA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 10 x ANP
Ligand MG 10 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.2Å
Relevant EMDB volumes: EMD-1980
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Bacterial actins and their diversity.
Ozyamak et al. (2013)
19 more

4 mentions without citation

A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.
Gayathri et al. (2012)
3 more