4a76

X-ray diffraction
1.92Å resolution

The Lin28b Cold shock domain in complex with heptathymidine

Released:
Source organism: Xenopus tropicalis
Primary publication:
The Lin28 cold-shock domain remodels pre-let-7 microRNA.
Nucleic Acids Res 40 7492-506 (2012)
PMID: 22570413

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-110086 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein lin-28 homolog B Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 90 amino acids
Theoretical weight: 9.94 KDa
Source organism: Xenopus tropicalis
Expression system: Escherichia coli
UniProt:
  • Canonical: B4F6I0 (Residues: 27-114; Coverage: 35%)
Gene names: LIN-28, csdd2, lin28b
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins
5'-D(*TP*TP*TP*TP*TP*TP*TP)-3' Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 7 nucleotides
Theoretical weight: 2.08 KDa
Source organism: Xenopus tropicalis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY
Spacegroup: P212121
Unit cell:
a: 51.61Å b: 77.575Å c: 100.231Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression systems:
  • Escherichia coli
  • Not provided