› 4a7e

X-ray diffraction
1.86Å resolution

X-ray crystal structure of porcine insulin flash-cooled at high pressure

Released:
DOI: 10.2210/pdb4a7e/pdb
PDB entry 4a7e coloured by chain, front view.
PDB entry 4a7e coloured by chain, side view.
PDB entry 4a7e coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Fast high-pressure freezing of protein crystals in their mother liquor.
Burkhardt A, Warmer M, Panneerselvam S, Wagner A, Zouni A, Glöckner C, Reimer R, Hohenberg H, Meents A
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 495-500 (2012)
PMID: 22505429

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134237 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I213
Unit cell:
a: 77.1Å b: 77.1Å c: 77.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.176 0.218

Quick links

Citations

3 citation in other articles

Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering.
Roedig et al. (2016)
2 more

2 mentions without citation

Fast high-pressure freezing of protein crystals in their mother liquor.
Burkhardt et al. (2012)
1 more