› 4a9x

X-ray diffraction
1.79Å resolution

Pseudomonas fluorescens PhoX in complex with the substrate analogue AppCp

Released:
DOI: 10.2210/pdb4a9x/pdb
PDB entry 4a9x coloured by chain, front view.
PDB entry 4a9x coloured by chain, side view.
PDB entry 4a9x coloured by chain, top view.
Source organism: Pseudomonas fluorescens Pf0-1
Primary publication:
A complex iron-calcium cofactor catalyzing phosphotransfer chemistry.
Yong SC, Roversi P, Lillington J, Rodriguez F, Krehenbrink M, Zeldin OB, Garman EF, Lea SM, Berks BC
Science 345 1170-1173 (2014)
PMID: 25190793

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional initiation protein Tat Chain: A
Molecule details ›
Chain: A
Length: 592 amino acids
Theoretical weight: 64.63 KDa
Source organism: Pseudomonas fluorescens Pf0-1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3K5N8 (Residues: 48-633; Coverage: 93%)
Gene name: Pfl01_5179
Sequence domains: Alkaline phosphatase PhoX

Ligands and Environments

4 bound ligands:
Ligand EDO 2 x EDO
Ligand ACP 1 x ACP
Ligand CA 3 x CA
Ligand FEO 1 x FEO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 70.4Å b: 78.9Å c: 111.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.287
Expression system: Escherichia coli BL21

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Citations

4 review citations

Microbial Surface Colonization and Biofilm Development in Marine Environments.
Dang et al. (2016)
3 more