› 4ab1

X-ray diffraction
2.2Å resolution

Recombinant Human Carboxylesterase 1 from whole Cabbage Loopers

Released:
DOI: 10.2210/pdb4ab1/pdb
PDB entry 4ab1 coloured by chain, front view.
PDB entry 4ab1 coloured by chain, side view.
PDB entry 4ab1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of recombinant human carboxylesterase 1 isolated from whole cabbage looper larvae.
Greenblatt HM, Otto TC, Kirkpatrick MG, Kovaleva E, Brown S, Buchman G, Cerasoli DM, Sussman JL
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 269-72 (2012)
PMID: 22442219

Function and Biology Details

Reactions catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
A steryl ester + H(2)O = a sterol + a fatty acid
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Liver carboxylesterase 1 Chain: A
Molecule details ›
Chain: A
Length: 532 amino acids
Theoretical weight: 58.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P23141 (Residues: 21-553; Coverage: 97%)
Gene names: CES1, CES2, SES1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand SCN 3 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6322
Unit cell:
a: 114.843Å b: 114.843Å c: 177.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.236
Expression system: Trichoplusia ni

Quick links

Citations

2 citation in other articles

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Xue et al. (2020)
1 more

3 mentions without citation

A molecular dynamics study of the formation, stability, and oligomerization state of two designed coiled coils: possibilities and limitations.
Piñeiro et al. (2005)
2 more