› 4abx

X-ray diffraction
2.04Å resolution

Crystal structure of Deinococcus radiodurans RecN coiled-coil domain

Released:
DOI: 10.2210/pdb4abx/pdb
PDB entry 4abx coloured by chain, front view.
PDB entry 4abx coloured by chain, side view.
PDB entry 4abx coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair.
Pellegrino S, Radzimanowski J, de Sanctis D, Boeri Erba E, McSweeney S, Timmins J
Structure 20 2076-89 (2012)
PMID: 23085075

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein RecN Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 175 amino acids
Theoretical weight: 18.56 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9WXF2 (Residues: 196-364; Coverage: 30%)
Gene names: DR_1477, recN
Sequence domains: RecF/RecN/SMC N terminal domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 73.21Å b: 43.97Å c: 133.63Å
α: 90° β: 97.71° γ: 90°
R-values:
R R work R free
0.203 0.201 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

An Overview of the Molecular Mechanisms of Recombinational DNA Repair.
Kowalczykowski SC. (2015)
4 more

4 mentions without citation

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges.
Timmins et al. (2016)
3 more