› 4adn

X-ray diffraction
1.65Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FusB protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 26.31 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8GNY5 (Residues: 1-213; Coverage: 100%)
Gene names: far1, fusB
Sequence domains:
Structure domains: Monooxygenase

Ligands and Environments

6 bound ligands:
Ligand ZN 2 x ZN
Ligand NA 8 x NA
Ligand CL 1 x CL
Ligand IMD 2 x IMD
Ligand GOL 3 x GOL
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 75.02Å b: 122.41Å c: 52.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.202
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Ribosome-targeting antibiotics and mechanisms of bacterial resistance.
Wilson DN. (2014)
4 more

2 mentions without citation

Structure and function of FusB: an elongation factor G-binding fusidic acid resistance protein active in ribosomal translocation and recycling.
Guo et al. (2012)
1 more