› 4aeq

X-ray diffraction
1.89Å resolution

Crystal structure of the dimeric immunity protein Cmi solved by direct methods (Arcimboldo)

Released:
DOI: 10.2210/pdb4aeq/pdb
Model geometry
Fit model/data
PDB entry 4aeq coloured by chain, front view.
PDB entry 4aeq coloured by chain, side view.
PDB entry 4aeq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The crystal structure of the dimeric colicin M immunity protein displays a 3D domain swap.
Usón I, Patzer SI, Rodríguez DD, Braun V, Zeth K
J Struct Biol 178 45-53 (2012)
PMID: 22366279

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-M immunity protein Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18002 (Residues: 44-141; Coverage: 70%)
Gene name: cmi
Sequence domains: YebF-like protein

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 66.058Å b: 83.552Å c: 38.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.
Ghequire et al. (2014)
10 other articles

6 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
5 more