› 4af1

X-ray diffraction
2Å resolution

Archeal Release Factor aRF1

Released:
DOI: 10.2210/pdb4af1/pdb
PDB entry 4af1 coloured by chain, front view.
PDB entry 4af1 coloured by chain, side view.
PDB entry 4af1 coloured by chain, top view.
Source organism: Halobacterium salinarum R1
Entry authors: Karlsen JL, Kjeldgaard M

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide chain release factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 46.69 KDa
Source organism: Halobacterium salinarum R1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B0R748 (Residues: 1-416; Coverage: 100%)
Gene names: OE_3973F, prf1
Sequence domains:
Structure domains: eRF1 domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 131.02Å b: 31.99Å c: 111.15Å
α: 90° β: 113.47° γ: 90°
R-values:
R R work R free
0.255 0.254 0.279
Expression system: Escherichia coli BL21

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